Transcription Factor Dynamics

Schematic of P53 protein bound to DNA. Image Credits: PDB101.

Transcription factor is a protein inside the cell regulating gene expression by binding to a specific DNA sequence.  It’s involved in cell response to environmental stimulus such as chemicals, irradiation and stress. NF-κB protein family, which includes p50 and p65 proteins, are the most common ones found in almost all animal cell types. The Estrogen Receptor is another one. Their activities are essential in cancer mechanism and cell apoptosis. We’re interested in studying the distribution and transportation of these proteins, as well as how they respond to the stimulus in living cells, to further uncover their signaling pathways. We especially want to observe their activities in single molecular level by using our fluorescent microscopy and intracellular labeling technique.

  1. Kai Wen Teng, Yuji Ishitsuka, Pin Ren, Yeoan Youn, Xiang Deng, Pinghua Ge, Sang Hak Lee, Andrew S. Belmont, and Paul Selvin. “Labeling proteins inside living cells using external fluorophores for microscopy”, eLife, 2016;5:e20378. Accepted November 21, 2016. DOI: 10.7554/eLife.20378.001
  2. Zhang, Ruobing; Fruhwirth, Gilbert; Coban, Oana; Barrett, James; Burgoyne, Thomas; Lee, Sang Hak; Simonson, Paul; Baday, Murat; Kholodenko, Boris; Futter, Clare; Ng, Tony; Selvin, Paul R. “Probing the Heterogeneity of Protein Kinase Activation in Cells by Super-Resolution Microscopy.” ACS Nano, Oct 21, 2016. 10.1021/acsnano.6b05356